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Danaher Inc
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Gallus BioPharmaceuticals
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Clonegene
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TIB MOLBIOL
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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Critical Role of 7,8-Didemethyl-8-hydroxy-5-deazariboflavin for Photoreactivation in Chlamydomonas reinhardtii
doi: 10.1074/jbc.m110.146050
Figure Lengend Snippet: FIGURE 3. Engineering a full-length PHR1 cDNA. Indicated by thin black lines are products generated by 5-RACE, 3-RACE, and RT-PCR that were used to form the full-length PHR1 cDNA. An N-terminal cassette was formed by joining products A and B using a NotI site. A C-terminal cassette was formed by joining products G, H, and F using SphI and EagI. The N- and C-terminal cassettes were joined to form the full-length PHR1 cDNA using a unique (*) SmaI site.
Article Snippet: 3 -RACE—Poly(A)RNA fromChlamydomonas strain cc-125 was reverse-transcribed using
Techniques: Generated, Reverse Transcription Polymerase Chain Reaction